Table 1.
Nucleotide sequences of the primers used for RT-PCR
| Protein (GeneBank#) | Primer | Product size | Sequence (5′ to 3′) |
|---|---|---|---|
| Human kv7.1 | Forward | 500 bp | CAGCCTGCACTTGGGGGCTC |
| (nm_000218.2) | Reverse | CCCACGCACAGTGGGCTCAG | |
| Human Nav1.7 | Forward | 446 bp | GCTCCGAGTCTTCAAGTTGG |
| (Nm_002977.3) | Reverse | GGTTGTTTGCATCAGGGTCT | |
| Human TASK2 | Forward | 567 bp | CTGCTCACCTCGGCCATCATCTTC |
| (Nm_003740.3) | Reverse | GTAGAGGCCCTCGATGTAGTTCCA | |
| Human TASK4 | Forward | 169 bp | ATAGCTGCACCCAGCCTCTA |
| (Nm_031460.3) | Reverse | GCATCTGGAACCTTCTGCTC | |
| Human TRPC4 | Forward | 317 bp | GCTGCTGGCATCTCGCTGGT |
| (Nm_016179.2) | Reverse | TGAAGCCCAGGACCCACGGT | |
| Human TRPC6 | Forward | 654 bp | CCGGCGGCAGACAGTTCTCC |
| (Nm_004621.5) | Reverse | GCCGGACTTGCCAGGCCTTT | |
| Human TRPM2 | Forward | 624 bp | CCCGGCACCTCCTCTACCCC |
| (Nm_003307.3) | Reverse | GGAAGTGGACGCTGACGGCC | |
| Human TRPV2 | Forward | 573 bp | GAGAACCCACACCAGCCCGC |
| (Nm_016113.4) | Reverse | GGCGGCCTGCTTCTTCAGGG | |
| Human Kv1.3 | Forward | 626 bp | CAGAGCATCGCGGCTTTGGCT |
| (Nm_00232.3) | Reverse | GCGGCCCCCGGACTGATAGT | |
| Human GAPDH | Forward | 323 bp | TCTTTTGCGTCGCCAGCCGAG |
| (Nm_002046.3) | Reverse | ACGTACTCAGCGCCAGCATCG | |