Fig. 1. Expression of AtSUMO1 in Arabidopsis. (A) Structure of the binary vector used for expression of AtSUMO1 in transgenic Arabidopsis. The 6xHis-3xFlag fused AtSUMO (HFAtSUMO1) were placed between the CaMV35S promoter and the OCS terminator of the pCAMBIA1300PT-derived binary vector. (B) Expression of HFAtSUMO1 in homozygous T3 over-expressing transgenic plants and control plants (Col-0 and HF inserted vector). The resulting crude extracts (40 μg) were subjected to SDS-PAGE, and immunoblot analysis was carried out with anti-HA antibody. (C) Identification of AtSUMO1 protein by MALDI-TOP MS. MALDI-TOP mass spectrometry-based analysis identified the peptide sequence of the expressed protein as AtSUMO1.