Fig. 2. PKR-dependent anti-HBV activity of IFN-α. Control and stable PKR knock-down (PKR-si) Huh-7 cells were transfected with pHBV1.2 and treated for four days with 100 or 500 U/ml of IFN-α. (A) Viral DNA in the capsid was analyzed by Southern blotting. (B) Aliquots of viral capsid were separated by native agarose-gel electrophoresis and immunoblotted with anti-core antibody. Similar result was obtained from more than three repeated experiments. Representative blots were shown with quantified signal intensity indicated below the image.
