Fig. 4. Kinase-dependent antiviral activity of PKR. Control and PKR-si cells were co-transfected with pHBV1.2 and siRNA-insensitive PKR constructs either expressing the wild-type or the kinase-defective (K296R) enzymes. Cells were harvested in 48 h. (A) Viral capsid was separated by native agarose-gel electrophoresis and immunoblotted with anti-core antibody. A representative blot was shown with signal intensity indicated below the image. (B) Cellular proteins were immunoblotted with the antibodies against PKR (HA-tag), phospho-PKR, eIF2α, phospho-eIF2α (intensity indicated below), or Hsp70 (for a loading control).
