Fig. 1. Sequences and constructs for RNA interference for OsBAK1. (A) Phylogenetic analysis of SERK proteins in Arabidopsis and rice. Amino acid sequences encoded by AtSERK1 (At1g71830), AtSERK2 (At1g34210), AtBAK1/AtSERK3 (At4g33430), AtSERK4/ BAK7/BKK1 (At2g13790), AtSERK5 (At-2g13800) in Arabidopsis and OsSERK1 (Os08g0174700), OsBISERK1 (AY463361), OsSERK2 (Os04g0457800), OsSERK3 (Os- 06g0225300), OsSERK4 (Os02g- 0283800) in rice (Oryza sativa) were used for CLUSTAL 2.1 multiple sequence alignment. Phylogenetic trees were generated by PHYML and MEGA (Guindon and Gascuel, 2003). (B, C) The nucleotide (B) and amino acid (C) sequences of a partial kinase domain encoded by OsSERKs for RNA interference for OsBAK1. Alignments were drawn by the ClustalW program. (D) RT-PCR analysis showing reduced expression of OsBAK1 by OsBAK1RNAi in several independent transgenic lines. (E) RT-PCR analysis showing reduced expression of ORK1 in the OsBAK1RNAi#5 and OsBAK1RNAi#11 transgenic rice plants.