Fig. 2. Binding of IQ motifs of IQGAP1 to 3xFLAG-hCaM in HEK 293T cells. CaM binding of overexpressed IQ motifs of IQGAP1 fused to EGFP in HEK293T cells using 3xFLAG-hCaM IP. EGFP-IQ(2-3) showed obvious apoCaM binding. EGFP-IQ(3-4) showed significant Ca2+/CaM binding in 3xFLAG-hCaM IP. However, EGFP-IQ(1-2), EGFP-IQ2, EGFP-IQ3 and EGFP-IQ4 displayed no CaM binding in 3xFLAG-hCaM IP. Bound proteins were visualized by immunoblotting using an anti-GFP antibody (upper) or using an anti-FLAG antibody (lower).
