Fig. 4. The LRR region of TTR1/ttr1 determines ecotype-specific responses to TRSV. (A) Multiple sequence alignments of the LRR regions of TTR1/ttr1 loci from multiple Arabidopsis ecotypes, separating A. thaliana ecotypes into two groups. (B) Schematic representation of TTR1, a “TNL”-type gene with five exons, and point mutations used to determine the role of TTR1 in causing LSN. (C) Each mutated clone (TTR1M) was introduced into Col-0 by Agrobacterium-mediated transformation. Plants transformed with L956S and/or K1124Q showed tolerance to TRSV, indicating the loss of TTR1 function. Scale bars = 1 cm.
