Fig. 2.
Heterologous expression of the ASR1 gene in transgenic yeast cells. (A) Schematic diagram showing the over-expression of ASR1 in yeast. ADHp, alcohol dehydrogenase promoter; 6XH, six histidine-tagged residue; OsASR1, ASR1 gene of Oryza sativa (rice); ADHt, alcohol dehydrogenase terminator; AmpR, selectable marker. Arrows indicate the direction of each gene. (B) Confirmation of ASR1 gene expression by semi-quantitative RT-PCR. The PCR product of the PDA1 gene was used as a standard control. (C) Expression of the ASR1 protein was measured by Western-blot analysis using an anti-His tag antibody. An anti-Tubulin (Tub) antibody was used as a housekeeping control. WT, yeast cells with empty vector alone; TF, ASR1-expressing transgenic yeast cells.