Fig. 1.
Induction of demethylation by Aza in MCF-7 cells. The methylation status of CpG sites at the SULT1A1 promoter was determined by sequencing after subcloning of the bisulfate-PCR product into the pGEM-T plasmid. Schematic diagram of the CpG sites at the promoter is drawn on the top. Methylated and unmethylated CpGs are indicated as black and open circles, respectively.