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. 2012 Jan 2;33(2):127–133. doi: 10.1007/s10059-012-2182-8

Fig. 1.

Fig. 1.

Induction of demethylation by Aza in MCF-7 cells. The methylation status of CpG sites at the SULT1A1 promoter was determined by sequencing after subcloning of the bisulfate-PCR product into the pGEM-T plasmid. Schematic diagram of the CpG sites at the promoter is drawn on the top. Methylated and unmethylated CpGs are indicated as black and open circles, respectively.