Skip to main content
. 2012 Apr 17;33(5):479–486. doi: 10.1007/s10059-012-2266-5

Fig. 2.

Fig. 2.

pLTA inhibited rhTNF-α-mediated IL-8 expression in HT-29 cells. (A) HT-29 cells were stimulated with the indicated doses of pLTA for 20 h followed by restimulation with 50 ng/ml of rhTNF-α for 24 h. IL-8 expression was analyzed from culture supernatant using ELSIA. (B) After stimulation with 50 ng/ml of rhTNF-α following pLTA pretreatment at the indicated dose, the cell survival rate was analyzed by a Premix WST-1 cell proliferation assay system (Takara Bio Inc.). (C) Cells were pretreated with the indicated ligands for 20 h and 50 ng/ml of rhTNF-α was retreated for 24 h. IL-8 expression was analyzed with supernatant using ELISA. The data are given as means ± standard deviations for triplicate samples in a single experiment. *, p < 0.05; **, p < 0.01 compared to 0 μg/ml of pLTA treatment (A) or rhTNF-α treatment only (C). UT indicates untreated cells.