Table 1.
Tagging vector | Number of lines tested | Number of lines bearing transposon
|
|
---|---|---|---|
Tn5393 (%) | Tn5563 (%) | ||
pGA2707 | 37 | 5 (13.5) | 1 (2.7) |
pGA2715 | 232 | 48 (20.7) | 5 (2.2) |
pGA2717 | 33 | 12 (36.4) | 0 (0.0) |
pGA2772 | 29 | 21 (72.4) | 1 (3.4) |
| |||
Total | 331 | 86 (26.0) | 7 (2.1) |
PCR was performed with f and g primers for Tn5393 and 5563a and 5563b primers for Tn5563.