Fig. 4.

Interaction of AtTCTP with microtubules. (A) In vitro protein-protein interaction between AtTCTP and tubulins. Purified GST-AtTCTP and porcine tubulin proteins were used for the pull-down assays. Precipitates were separated on SDS-PAGE gels, and immunodetection was performed with anti-β-tubulin and anti-GST antibodies. The amount of tubulin added in each assay was also shown as a control (i.e., Tubulin control). (B) Relative amounts of AtTCTP-bound tubulin at 37°C or 4°C compared to the amount of tubulin added in the reactions (i.e. [Tubulin in precipitates]/[Tubulin in Tubulin control]). Each assay was repeated three times. Error bar = SD (n = 3).