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Different ALP mRNA expression levels according to week of age. Primary calvarial cells were isolated from the SmoM2;OCNCre mutant or the control at various postnatal stages (2–5 weeks). The cells were incubated in the presence or absence of DAG followed by an ALP mRNA expression assay. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the control cultures in GM. #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. the control cultures in OIM.
