Table 1.

The comparison of cell survival ratio of GSK-3β wt with its PY mutants

GSK-3 β	Rate of apoptosis (%) by FACS
WT	25 +/− 3
R111A	10 +/− 4
Y117A	11 +/− 5
K292R	23 +/− 4
(Vector only)	7 +/− 2
	Mean value of 5 repeats

HA-GSK-3β WT, its PY mutant (R111A, Y117A), K292R mutant or pcDNA 3.0 was transfected and the rate of apoptosis measured by FACS. HA-GSK-3β PY mutant (R111A, Y117A), which was not dominantly localized into the nuclear was less effective on the cell apoptosis, compared to GSK-3β WT constructs. The apoptotic effect of GSK-3β PY mutant (R111A or Y117A) was reduced to 2.5 times that of GSK-3β WT.