Fig. 6.

HS-1793 down-regulated mitochondrial biogenesis regulatory proteins. (A) Western blot data showed that 140 μM resveratrol treatment did not change the levels of mitochondrial biogenesis regulatory proteins, including TFAM and TUFM, in MCF-7 cells. (B) HS-1793 (60 μM) treatment significantly down-regulated the protein expression levels of both TFAM and TUFM. Real-time PCR data indicated that TFAM (C) and TUFM (D) were also significantly decreased by HS-1793 treatment. (E) Resveratrol (140 μM) or 60 μM of HS-1793 were added to MCF-7 cells for 72 h and the mitochondrial DNA ratio was measured using real-time PCR. HS-1793 treatment decreased the mitochondrial DNA ratio by 50% (***P < 0.005).