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. 2012 Mar 23;33(4):335–341. doi: 10.1007/s10059-012-2287-0

Fig. 1.

Fig. 1.

Expression of hBD-2 in HIV-1 Tat-stimulated cells. RPMI 8226 cells were treated with HIV-1 Tat (500 ng/ml) for the indicated time or at various concentrations of Tat for 12 h. The total RNA and protein in the culture supernatants were analyzed by RT-PCR (A, B) and ELISA (C, D), respectively. (E) Activation of hBD-2 promoter by HIV-1 Tat. The transfected RPMI 8226 cells with phBD-2-Luc were exposed to HIV-1 Tat for 24 h and the luciferase activity was measured. (F) Effect of metabolic inhibitors on HIV-1 Tat-induced hBD-2 expression. RPMI 8226 cells pretreated with actinomycin D (10 μg/ml) or cyclohexamide (10 μM) for 1 h were exposed to HIV-1 Tat. Expression of hBD mRNA and protein was analyzed by RT-PCR and ELISA, respectively.