Fig. 1.

Sequence analysis of CaSD1. (A) Sequence alignment of CaSD1 in C. annuum L. cv. ‘Bukang’ and ‘CM334.’ Black box denotes sequence match between ‘Bukang’ and ‘CM334.’ (B) Schematic structure of CaSD1 genomic DNA region and amino acid sequence of CaSD1. The black line indicates the 5′ and 3′ untranslated regions and introns. The box with diagonal line indicates exons of CaSD1. Black arrow designates primers for PCR amplification of the repeat region. Black box: signal peptide, light gray: non-repeat region, white: repeat region, dark gray: another non-repeat region. n.t, nucleotide. a.a, amino acid. (C) Four genomic DNA samples from each Capsicum species were used for PCR amplification. PCR products were electrophoresed in 1% agarose gels, stained with ethidium bromide, and photographed under ultraviolet light.