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Northwestern blot analysis to confirm interaction of PVX CP with SL1 RNAs. (A) One-DE analysis of extracted S100 and recombinant PVX CP. Proteins were stained with Coomassie Brilliant Blue after electrophoresis. The arrow indicates PVX CP recombinant protein (about 30 kDa). One-DE Northwestern blot analysis using 32P-labeled SL1(+) RNA transcripts (B) and 32P-labeled SL1(−) RNA transcripts (C). (D) Two-DE analysis of extracted S100 proteins visualized by silver staining.
