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EMSA to confirm the interactions of three N. benthamiana proteins and SL1 RNAs. In each image, only 32P-labeled RNAs were loaded in the first lane indicated by ‘R’ whereas 0.5 μg and 2 μg of each purified protein were loaded in lanes 2 and 3, respectively. The top arrow indicates RNA-protein complexes while the bottom arrow indicates labeled SL1 RNAs.
