Table 1.
Host proteins that bind to PVX SL1 RNAs as determined by proteomics
| Spot # | Accession # | Protein name | MW Da/pI | SL1 strand | No. of peptide | Seq. Cov. % | MOWSE score | Accession # of Arabidopsis | E-value | Function |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 20086364 | Putative multiprotein bridging factor 1 | 15327/10.0 | (−) | 6 | 30.7 | 1856 | AT3G58680 | 7.00E-45 | TF |
| 2 | 1841462 | Elongation factor 2 | 19875/5.3 | (−) | 5 | 38 | 193 | AT1G56070 | 7.00E-97 | SP |
| 3 | 90654964 | DnaJ heat shock protein | 20774/9.3 | (−) | 6 | 31.6 | 788 | AT1G77930 | 4.00E-63 | HSP |
| 4 | 5932420 | LIM domain protein WLIM2 | 20812/5.6 | (+)(−) | 10 | 35 | 2389 | AT3G55770 | 2.00E-75 | TF |
| 5 | 5932413 | LIM domain protein WLIM1 | 21521/9.1 | (+)(−) | 6 | 30.6 | 745 | AT1G10200 | 8.00E-83 | TF |
| 6 | 3256375 | Heat shock protein 26 (Type I) | 22574/5.2 | (+)(−) | 5 | 33.3 | 381 | AT4G27670 | 2.00E-37 | HSP |
| 7 | 15778156 | 14-3-3 protein | 22981/5.6 | (+)(−) | 7 | 29.4 | 1149 | AT4G09000 | 2.00E-90 | SP |
| 8 | 417063 | Floral homeotic protein GLOBOSA | 24691/8.9 | (+) | 5 | 29.7 | 231 | AT5G20240 | 5.00E-51 | TF |
| 9 | 6552359 | Myb-related transcription factor LBM1 | 31985/5.1 | (−) | 6 | 30.2 | 1141 | AT3G23250 | 6.00E-71 | TF |
| 10 | 1732247 | Transcription factor Myb1 | 32005/5.5 | (−) | 4 | 22.3 | 258 | AT3G23250 | 9.00E-68 | TF |
| 11 | 5821136 | Heat shock factor | 32106/6.0 | (+) | 4 | 15.1 | 84.9 | AT4G36990 | 2.00E-65 | HSP |
| 12 | 34811738 | Potyviral CP interacting protein 2a | 33872/9.3 | (+) | 5 | 23 | 592 | AT1G10350 | 1.00E-101 | HSP |
| 13 | 34811740 | Potyviral CP interacting protein 2b | 33930/9.3 | (+) | 5 | 16.4 | 94 | AT1G59725 | 1.00E-104 | HSP |
| 14 | 74038607 | Tobamovirus multiplication 3 | 34023/9.8 | (−) | 4 | 28.9 | 315 | AT2G02180 | 1.00E-114 | TF |
| 15 | 4760596 | DNA-binding protein NtWRKY3 | 35940/9.6 | (+) | 6 | 15.5 | 3101 | AT4G31550 | 3.00E-75 | TF |
| 16 | 19716176 | TMV-MP30 binding protein 2C | 35998/5.0 | (+)(−) | 5 | 21.2 | 102 | AT5G08120 | 7.00E-34 | CSP |
| 17 | 57014014 | Ribosomal protein L2 | 36644/10.9 | (+) | 11 | 28.1 | 756 | ATMG00560 | 1.00E-129 | RBP |
| 18 | 6179940 | DnaJ-like protein | 37117/9.1 | (−) | 4 | 27.5 | 188 | AT2G20560 | 1.00E-105 | HSP |
| 19 | 39598908 | CMV 1a interacting protein 1 | 41538/6.4 | (+) | 7 | 27.9 | 5836 | AT1G23360 | 2.00E-05 | SP |
| 20 | 78096654 | Mitogen-activated protein kinase | 42884/6.1 | (−) | 6 | 24.3 | 167 | AT4G01370 | 0 | SP |
| 21 | 18143321 | Wound induced protein kinase | 42898/5.2 | (−) | 7 | 28 | 795 | AT3G45640 | 0 | SP |
| 22 | 51458330 | Trehalose-phosphate phosphatase | 42945/7.6 | (−) | 10 | 31.4 | 2612 | AT5G51460 | 1.00E-150 | SP |
| 23 | 11133532 | Shaggy-related protein kinase NtK-1 | 46309/8.6 | (−) | 7 | 18.3 | 377 | AT5G26751 | 0 | SP |
| 24 | 1617200 NSK6; | Shaggy-like kinase6 | 53434/7.9 | (−) | 10 | 20.8 | 6019 | AT4G00720 | 0 | SP |
| 25 | 70994254 | PVX CP | 25105/7.7 | (+)(−) | 5 | 16.5 | 269 |
1
Table headings: Spot #, protein spot number from Fig. 3; Accession #, accession number of N. tabacum proteins from NCBI; MW Da/pI, theoretical Daltons/isoelectric point; No. of peptide, number of peptides matched to the identified protein sequences; Seq. Cov. %, the percent coverage for the protein based upon the matched peptide sequences; MOWSE score, peptide identification score; E-value, expectation value between candidate proteins of N. tabacum and Arabidopsis
2
TF, transcriptional factor; HSP, heat shock protein; SP, signaling protein; CSP, cytoskeletal protein; RBP, rRNA binding protein