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Effects of different concentrations of nicotine on the mRNA expression of nAChRs. (A) The mRNA expression of α1–7 and β1–4 nAChRs in PDLSCs was analyzed using RT-PCR. Cells were incubated with different concentrations of nicotine (0–10−2 M) and the mRNA levels of (B) α7 and (C) β4 nAChRs were then determined by real time RT-PCR technique. The values reported are the mean ± S.D. of three independent experiments. *P < 0.05 or #P < 0.001 vs. control value.
