Table 1.
List of primers of the nAChR subunit genes
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | Size (bp) |
|---|---|---|---|
| α1 | GGCTCCGAACATGAGACCCG | GCGTGACTTTGGGAGTTCCTTT | 234 |
| α2 | TGACCCACATGACCAAGGCCCA | TGGTGAACAGCAGGTACTCGCC | 536 |
| α3 | CCGAGGCCCCTCTACGGT | CACACAGCTTAGTGCTTA | 428 |
| α4 | CCTCGGCCTGTCCATCGCTCA | AAGACGGTGAGCGACAGCAGC | 668 |
| α5 | CCATCATCTTCAAAAGTCATA | CCCATTTATAAATAACAGGAAC | 453 |
| α6 | GCTGTGCAACTGAGGA | AAGACGGGTGAGC | 921 |
| α7 | CCCGGCAAGAGGAGTGAAAGGT | CCGGGCCTCTTCATTCGCAG | 442 |
| β1 | GTGTCAGGGTCAGGGTTGGT | TGCGGCGGATGATGAGGTAG | 579 |
| β2 | GTGTCCTTCTATTCCAAT | AATGATGAAGTCATACGT | 305 |
| β3 | AAGGGGAACAGAAGGGACGG | GAAGCAGTACGTCGCGGACG | 461 |
| β4 | CAACAACCTGATCCGCCCAGC | GAAGGGAAAGTACTTCACCTC | 366 |