Fig. 7.

PGE2 induce Raf and ERK phosphorylation. (A) Ras activity was assessed by immunoblotting samples for Ras immunoprecipitated form lysates using Raf-1 RBD. Densitometer measurements of Ras activity (units as pixels) are shown below the blot. (B) Phosphorylated Raf-1 and Erk were detected by immunoblotting analysis with antibody specific for phosphorylated Raf-1 and Erk (pRaf and pErk, respectively). Protein loading was determined by stripping and reprobing the same nitrocellulose membrane for total Raf-1 and Erk protein level. Results are representative of three independent experiments.