Fig. 2.

Dieckol suppresses migration and invasion of B16F10 cells by reducing intracellular ROS. (A) Dieckol attenuates intracellular ROS. B16F0 and B16F10 cells (4 × 10⁴ cells/well) were incubated in the presence or absence of 100 μM H₂O₂ for 48 h, followed by incubation with or without dieckol (25 μg/ml) or DPI (20 μM), a NADPH oxidase-dependent ROS-quenching agent, for 24 h. Cellular ROS levels were assessed by DCF-DA. All data are presented as mean ± SD. Statistical significance by Student’s t-test is shown. (B) Effects of H₂O₂, dieckol, and/or DPI on cell viability. Cells were treated with H₂O₂ (100 μM), dieckol (25 μg/ml), and/or DPI (20 μM) for 48 h and assessed using MTT assay. The results of 3 independent experiments were averaged. (C, D) Cells were treated as shown in (A). Wound-healing scratch assays and Matrigel invasion assays were performed for 16 h. All data are presented as mean ± SD. Statistical sig-nificance by Student’s t-test is shown.