Fig. 1.

Representative data of the MSP analysis of TMEFF2 gene in NSCLC lung tissues. The methylation status of the TMEFF2 gene was analyzed in tumor and non-malignant lung tissues. MSP was carried out using unmethylation-specific (U) and methylation-specific (M) primers. CpGenome™ Universal methylated and unmethylated DNA (Chemicon) was used as a positive control for the M and U forms. Water was used as a negative control. Lane U, amplified product obtained using U primers; Lane M, amplified product obtained using M primers; Pos, positive control; Neg, negative control.