Fig. 1.

Construction of retroviral vectors and bone marrow transduction. (A) Four retroviral vectors were constructed based on the MSCV (Clontech) backbone. GFP and truncated nerve growth factor receptor genes were used as markers for MYC and PIM2 expression respectively. In both cases, internal ribosome entry sites (IRES) were used to link the proto-oncogenes with their respective markers. (B) Immunoblotting result of protein extracts obtained from MYC or PIM2/MYC-transduced cells using the antibodies against MYC or PIM2. (C) Representative results of retroviral transduction of mouse bone marrow cells. PIM2 and MYC or GFP and MYC retroviral vectors were co-infected into fresh mouse bone marrow cells. After 3 days, the cells were harvested and stained for NGFR expression (redfluorescence, FL2), and analyzed for GFP and NGFR expression.