Skip to main content
. 2012 Jul 18;34(2):159–164. doi: 10.1007/s10059-012-0055-9

Fig. 1.

Fig. 1.

p190RhoGEF transgene expression in CD11c-expressing DCs. (A) A schematic view of p190RhoGEF transgene in the pDOI expression vector: a cassette vector for high-level expression driven by a hybrid invariant chain promoter, consisting of the promoter region spliced together with the enhancer elements (Eα) from the first intron of the Ii gene. Arrows indicate primer pairs used for the detection of the integrated transgene. (B) Transgene inclusion was analyzed by PCR on genomic tail DNA from non-TG, littermate (LtM), and two separate TG mice. (C) mRNA transcripts of p190RhoGEF in CD11c+ DCs isolated from the spleens of LtM and TG mice. mRNA transcripts of GAPDH were shown as loading controls. (D) Expression analysis of p190RhoGEF proteins in CD11c+ DCs and T cells isolated from the spleens of LtM and TG mice. Expression of β-actin was shown as loading controls. Data shown are representatives of five TG lines tested.