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. 2012 Jul 18;34(2):133–142. doi: 10.1007/s10059-012-2286-1

Fig. 1.

Fig. 1.

Knockdown of Myog decreases expression of late muscle genes in differentiating C2C12 myoblasts. Stable transfected cells were obtained after transfection of pSuper-Myog into C2C12 myoblasts and selection with G418. Total RNAs were extracted from the transfected cells and RT-PCR was performed to estimate the transcripts of Myog, MyoD, Myf5, early genes (Cdh15, Desmin), and late genes (Mhc, Mck, Tncs, Mylf3). GAPDH was used as an internal control. C2-V: C2C12 cells transfected with pSuper empty vector (control); C2-G1, 2 and 3: C2C12 cells transfected with pSuper-Myog.