Fig. 5.

Yeast trans-activation assay. (A) Schematic representation of the truncated C-terminal catalytic domain of AtMYB44. Various deletion constructs of the AtMYB44 protein were fused to the GAL4 DNA-binding domain in the yeast expression vector pGBK-T7. The proline-rich region in the activation domain is indicated by grey boxes. The two conserved motifs within the subgroup 22 MYB genes 22.1 and 22.2 are indicated by black boxes. Small letters under the bars represent amino acid residue numbers in the AtMYB44 protein. (B) Trans-activation activities of the truncated AtMYB44 protein. Quantitative analysis of β-galactosidase activity was conducted with o-nitrophenyl β-D-galactopyranoside as a substrate. Data are means and standard deviations (error bars) of four independent experiments with triplicate samples.