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R-III is incorporated into and induces phenotypic changes in HSCs. (A) 293 cells were stably transfected with expression vector for His-tagged albumin, RBP or His-tagged R-III and conditioned medium collected from each stable transfectant was analyzed by Western blotting. (B, C) HSCs after passage 2 were incubated with the conditioned media for 24 h and subjected to Western blotting (B) and oil red O staining (C).
