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. 2012 Aug 8;34(3):279–287. doi: 10.1007/s10059-012-0103-5

Fig. 2.

Fig. 2

Histone modifications within LHY, CCA1, and TOC1 chromatin in 12L12D. (A) ChIP assay of LHY chromatin with H3Ac-, H4K4me3-, H4Ac-, and H3K36me2-specific antibodies. WT seedlings were grown in 12L12D for 7 days and harvested at ZT0 and ZT12 (A-C). ZT means time (h) after lights on. “Input” indicates chromatin before immunoprecipitation. “Mock” refers to control samples lacking antibody. ACTIN 1 was used as a control chromatin. The gray boxes in the front indicate exonic 5′ untranslated regions (UTRs), and the rear gray boxes represent 3′ UTRs (AC). Labeled lines indicate regions amplified in ChIP-PCRs using specific primers (see “Materials and Methods”; A-C). (B) ChIP assay of CCA1 chromatin. (C) ChIP assay of TOC1 chromatin.