Fig. 1.

ROS generation in soybean root during NaCl treatment. (A) Soybean seedlings were treated with 300 mM NaCl for the indicated times, and then O₂⁻ generation was measured using XTT-formazan using a spectrophotometer at A₄₇₀. Mean ± SD of 2 samples. (B) Soybean seedlings were treated with 10 μM CM-H₂DCFDA for 60 min, followed by 300 mM NaCl treatment for the indicated times. H₂O₂ generation signals in secondary roots were detected using the FITC channel of a fluorescence microscope. (*) indicates H₂O₂ signal. Scale bar = 1 mm.