Chlorophyll content and ion leakage in H2O2-treated WT and osldc-like 1 rice plants. (A) Eight-week-old rice leaf segments were treated with H2O2 for 18 h and then chlorophyll content was measured. (B) Ion leakage in H2O2-treated WT and osldc-like 1 rice plants. Leaves were incubated in the presence (closed symbols) of 50 mM H2O2 for 48 h. Untreated controls (open symbols) were incubated in 20 mM MES buffer. Data represent means WT (○, •); HT mutant, (□, ▪); HM mutant, (▵, ▴). Data represents means. Differences between H2O2-treated osldc-like 1 and WT rice plants based on the two-tailed Student’s t-test are indicated by two asterisks (P < 0.01).