Fig. 4.
Phenotypic analyses of Lyar-mutant mice. (A) Macroscopic appearance of adult testes from a Lyargt/gt mouse and a WT littermate (Lyar+/+). Scale bar = 3 mm. (B) Comparison of the testis-weight-to-body-weight ratio in Lyar+/+ (n = 13) and Lyargt/gt mice (n = 14). Testes were trimmed of fat and weighed. Values are means ± SEMs. The average testis-weight-to-body-weight ratio for WT and Lyargt/gt males was 0.67 ± 0.03 and 0.64 ± 0.04%, respectively. Histological analyses of testes from adult Lyar+/+ mice (C) and Lyargt/gt mice (D). Histological sections of seminiferous tubules obtained from the testes were stained with hematoxylin and eosin. Scale bar = 100 μm. (E) Morphologies of Lyar+/+ and Lyargt/gt sperm. Sperm were prepared from the cauda epididymis and vas deferens and observed under a light microscope. Scale bar = 100 μm. (F) Number of mature sperm from Lyar+/+ (n = 9) and Lyargt/gt mice (n = 10). Values are means ± SEMs. The average number of mature sperm in WT and Lyargt/gt males was 2.40 ± 0.46 × 107 and 2.86 ± 0.32 × 107, respectively.