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. 2013 Jan 9;35(1):79–86. doi: 10.1007/s10059-013-2282-0

Fig. 5.

Fig. 5.

Effects of calphostin C, NS-398, or thapsigargin on S1P-induced actions in cultured ICC from mouse small intestine. (A) Pre-treatment with calphostin C (100 nM) did not result in blockade of S1P (1 μM)-induced actions at a holding potential of −70 mV. (B) Pre-treatment with NS-398 (10 μM) did not result in blockade of S1P (1 μM)-induced actions at a holding potential of −70 mV. (C) Pre-treatment with thapsigargin (5 μM) resulted in blockade of S1P (1 μM)-induced actions at a holding potential of −70 mV. Responses to treatment with calphostin C, NS-398, or thapsigargin on S1P-induced actions are summarized in (D, E, and F) (n = 4). Bars represent mean values ± SE. *(P < 0.05) Significantly different from S1P alone. Dotted lines indicate the zero current levels. Con, control; CC, calphostin C; NS, NS-398; TSG, thapsigargin.