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Effects of S1P on external Ca2+-free condition and on intracellular Ca2+ oscillation in cultured ICC from mouse small intestine. (A) Pre-treatment with Ca2+-free solution resulted in inhibition of S1P (1 μM)-induced actions at a holding potential of −70 mV. (B) Fluo-4/AM was used for monitoring changes in the intracellular Ca2+ concentration.
