Skip to main content
. 2012 Nov 29;35(1):61–69. doi: 10.1007/s10059-013-2273-1

Fig. 4.

Fig. 4.

NonO functions as a transcriptional activator of oct4 gene expression. (A) Transcriptional effect of NonO on the oct4 promoter was determined with a luciferase assay. A NonO expression vector (PCNA-NonO) and a pGL3-F luciferase vector containing the NonO recognition sequence were constructed and transfected into 293T cells in various combinations (NonO−:F−, NonO+: F−, NonO−:F+ and NonO+:F+). Student’s t-test was used for the statistical analysis. **p < 0.01 vs. F−:NonO− (n = 6). (B, C) Positive regulation of oct4 gene expression by NonO. An siRNA against NonO and a control siRNA were constructed and transfected into ES cells, and oct4 expression was determined 24 h after siRNA treatment. (B) Quantitative RT-PCR analysis of the NonO and oct4 mRNA levels in the control and NonO-siRNA treated cells. Student’s t-test was used for the statistical analysis. **p < 0.01 vs. ConSiRNA (n = 6). (C) Immunocytochemical analysis of NonO and oct4 (green color: NonO, red color: oct4, blue color: DAPI). Red arrows show the co-expression of NonO and oct4 in the same cells, and white arrows show the inhibition of oct4 in the NonO-siRNA treated cells.