Fig. 5.

Immunofluorescence staining of cell phenotypes cultured on the amino modified surfaces and PLL surfaces. Nestin immunofluo-rescence stained for NSCs after one day of culturing; GFAP, β-tubulin III and O₄ immunofluorescence double staining of NSCs differentiation showed astrocytes, neurons and oligodendrocytes after seven days of culturing. Blue indicates DAPI staining of nuclei. Scale bar (A–D), 100 μm; scale bar (A′B′C′D′ and A″B″C″D″), 50 μm. (A–D), amino modified surfaces with different densities; (Control), PLL coated surfaces.