Fig. 3.

Curcumin modulates apoptotic proteins at protein and mRNA level. (A) Western blot analysis of caspase-3 activation and p53 and Prp4B expression in HCT-15 cells. HCT-15 cells were treated with 30 μM of curcumin and whole-cell lysates were extracted from cells and separated by SDS-PAGE and the resulting proteins were detected by immunoblotting. (B) HCT-15 cells were treated with 50 μM of curcumin and whole-cell lysates were extracted from cells and separated by SDS-PAGE and the resulting proteins were detected by immunoblotting (C) RTPCR analysis of Prp4B and p53. HCT-15 cells were treated with 30 μM of curcumin and total RNA was isolated from cells, and RT-PCR was performed. PCR products were separated on 1% agarose gel and photographed. (D) HCT-15 cells were treated with 50 μM of curcumin and PCR products were separated on 1% agarose gel and photographed. Actin was also examined as a reference.