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Kifunensine alters the size and number of lysosomal punctae. SK-N-SH cells were preconditioned with kifunensine for 72 h. Treatment was performed with tunicamycin (1 μg/ml) and kifunensine treatment for 20 h. Immunofluorescent staining was performed for Cathepsin D (A) and LAMP1 (B). Nuclear counterstain was performed with DAPI. Scale bar indicates 10 μm. Quantifications of numbers, area, perimeter and circularity were performed as described in materials and methods. CathD, Cathepsin D.
