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pLTA reduces production of TNF-α and NO as well as expression of iNOS in macrophages. RAW264.7 cells were stimulated with 0.1 μg/ml LPS in the presence or absence of pLTA. (A) Levels of TNF-α were measured in culture supernatants by ELISA 12 h after treatment. (B) Production of nitrite in culture media was measured by Griess reagent 12 h after treatment. (C) Expression levels of iNOS were measured in cell lysates by Western blotting 12 h after treatment.
