Fig. 4.
pLTA inhibits adhesion of LPS-stimulated THP-1 cells to HUVEC. [C14]-acetate-labeled THP-1 cells were preincubated for 12 h with 10 μg/ml pLTA. Cells were washed twice with PBS and incubated with 0.1 μg/ml LPS for 12 h. THP-1 cells were added to the HUVEC monolayer and allowed to adhere for 1 h. (A) THP-1 cells attached to HUVEC were observed under a microscope at 400 X magnification. (B) Adhesion was measured as described in the “Materials and Methods”. (C) Effects of pLTA on TNF-α-induced mRNA expression of ICAM-1, VCAM-1, and E-selectin. HUVEC were pre-incubated with 10 μg/ml pLTA for 12 h and then stimulated with 2 ng/ml TNF-α for 6 or 12 h. Levels of mRNA for adhesion molecules were determined by RT-PCR. The results shown are from a single experiment and are representative of three separate experiments.