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Down-regulation of TLRs and chemokine receptors. THP-1 cells were treated with or without 10 μg/ml pLTA for 12 h, and then further treated with 0.1 μg/ml LPS for 24 h. mRNA expression of (A) TLR2 and TLR4 and (B) CCR2, CCR7, and CX3CR1 was examined by RT-PCR and visualized on a 0.8% agarose gel. The β-actin was used as an endogenous positive control.
