Fig. 4.

Hemolytic activity of moricin (A) and cecropin B (B). Freshly isolated porcine red blood cells were incubated with various concentrations of moricin and cecropin B (0–256 μg/ml) in 5 mM phosphate, pH 7.4, 5 mM glucose, 154 mM NaCl (open circles), or 3 g/l TSB, 287 mM glucose (closed circles), separately. Release of hemoglobin, as a measure of hemolysis, was measured at 450 nm. Release of hemoglobin upon addition of Tween-20 was set at 100 %. Data represent mean ± SD.