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. 2013 Feb 20;35(2):134–141. doi: 10.1007/s10059-012-2257-1

Fig. 2.

Fig. 2.

Mitochondrial biogenesis during 3T3-L1 adipocyte differentiation. (A) The relative amount of mtDNA was analyzed by real-time PCR during 3T3-L1 adipocyte differentiation. (n = 4; means and s.d.s are shown; *P < 0.05). Ctr, control; R, Rosiglitazone; EtBr, ethidium bromide. (B) Western blot analysis of OXPHOS subunits encoded by nDNA (NDUFA9, SDHA, SDHB, UQCRC2, COX4, and ATP5A1) and mtDNA (ND6) during 3T3-L1 adipocyte differentiation. (C) BN-PAGE analysis of assembled OXPHOS complexes in isolated mitochondria during 3T3-L1 adipocyte differentiation. (D) Western blot analysis of Crif1, VDAC and OXPHOS subunits encoded by nDNA (NDUFA9, SDHA, UQCRC2, COX4, and ATP5A1) and mtDNA (ND1) in isolated mitochondria during 3T3-L1 adipocyte differentiation.