Modulation of mitochondrial OXPHOS following treatment with rosiglitazone or EtBr. 3T3-L1 cells were treated with 1 μM rosiglitazone or 100 ng/ml EtBr. (A) Oil red O staining at day 7 of 3T3-L1 adipocyte differentiation. (B) Northern blot analysis of Crif1, Ppargamma, Cox1 and β-actin at days 0, 1, 3, 7 of 3T3-L1 adipocyte differentiation. (C) Western blot analysis of the OXPHOS complex subunits NDUFA9, SDHA, SDHB, UQCRC2, COX4 and ATP5A1, as well as OPA1, TOM40, and β-actin, at days 1, 3 and 7 of 3T3-L1 adipocyte differentiation.