Fig. 4.

Role of p38 MAPK and NF-κB on S100A8/A9-induced cell invasion and migration. Cell invasion (A, B) and migration (C, D) were measured in cells pretreated with 10 μM SB203580 (SB) and 5 μM Bay for 30 min using a transwell assay. These cells were added to upper chamber and the lower wells of the chamber were filled with medium containing 1 μg/ml S100A8/A9 (A89). After incubation for 18 h, migrated and invaded cells were quantified. Representative and quantitative data were shown. Data are mean ± SEM of three independent experiments. *P < 0.05 compared with control (C); #P < 0.05 compared with S100A8/A9 (A89) alone.