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The local environment of Tyr30 in KSI. (A) Tyr30 is fully buried in the active site as determined by solvent accessible surface area calculations (see “Materials and Methods”), and it is shown as a stick model (yellow). (B) The tyrosine residue at position 30 is surrounded by a number of hydrophobic groups including Ile26, Met29, Ala34, Val36, Ile51, Met101, Met109 and the aromatic moieties of both Tyr14 and Tyr55. The figure was generated by PyMOL.
