Fig. 4.

Relative mRNA levels of lec-1, lec-5, and nex-1 genes in N2 and daf-16 mutant worms in CN and CS. Wild-type N2 and daf-16(mu86) mutant worms were synchronized at L1 stage and grown in CN and CS conditions, and then young adult worms were collected to perform qRT-PCR as described in materials and methods. Relative mRNA level of each gene in each condition was defined as the mRNA level of each gene in each condition averaged from triplicate measurements of two independent experiments and normalized with that of act-1, the internal control. Then relative values are shown using CN N2 value as 1.0.