Distribution of Dlg and synaptic levels of GluRIIA are altered in tbc1d15-17 mutants. (A–E) Single confocal slices of NMJ 6/7 doubly stained with anti-HRP and anti-Csp (A), anti-NC82 (B), anti-Dlg (C, D), or anti-GluRIIA (E) antibodies in the indicated genotypes. Note that Dlg distribution and synaptic GluRIIA level are defective in tbc1d15-17d05023 (C) and BG57-GAL4/UAS-tbc1d15-17RNAi (D) larvae. (E) Levels of synaptic GluRIIA are increased in tbc1d15-17d05023 mutants compared with wild-type larvae. (F) Quantification of GluRIIA to HRP level ratio in the indicated genotypes. Note that levels of GluRIIA are significantly increased in BG57-GAL4/UAS-tbc1d15-17RNAi compared with the BG57-GAL4/+ control (*P < 0.001). Scale bars, 20 μm.